Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media

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Abstract
The effect of suspending media on the stability of pseudorabies virus [for vaccination of domestic animals] upon freeze-drying and subsequent storage was studied. A variety of media was tested including sodium glutamate, sucrose, lactose, lactalbumin hydrolysate, peptone, a combination of sucrose, dextran and glutamate, and various combinations of sucrose, glutamate and potassium phosphates. Suspending media containing glutamate, alone or in combination with sucrose and dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and upon storage. Some possible functions of these additives in preventing injury to the virus during freezing and drying are suggested.