Mitochondrial DNA of the yeastKluyveromyces: guanine—cytosine rich sequence clusters

Abstract
Mitochondrial DNA from the yeast Kluyveromyces marxianus var. lactis (K. lactis) is a circular molecule of 39 kilobase-pairs. A genetic and physical map was constructed. We found that this genome contained a large number of guanine-cytosine (GC)-rich sequence clusters, many of which are characterized by the presence of SacII restriction sites (CCGCGG). The primary sequence of the GC clusters often showed a palindromic structure. These GC clusters were present in several varieties of K. marxianus, but not in others. The presence of these clusters is a major feature that distinguishes K. lactis strains from those of K. marxianus var. marxianus (including K. fragilis).