Regulation of the Synthesis of Human Chorionic Gonadotrophin by 5-Bromo-2′-Deoxyuridine and Dibutyryl Cyclic AMP in Trophoblastic and Nontrophoblastic Tumor Cells

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Abstract
Synthesis of hCG and its α subunit (hCG-α) by trophoblastic tumor cell lines (JEG-3, Reid, and BeWo) and by nontrophoblastic tumor cell lines [ChaGo and three HeLa lines (HeLa CCL2, HeLa S3 and HeLa 65)] was studied. cAMP, dibutyryl cAMP, and 8-bromo-cAMP induced, but 5-bromo-2′-deoxyuridine inhibited, the synthesis of hCG-α and hCG in trophoblastic tumor cells. Addition of 5-bromo-2′-deoxyuridine partially blocked the induction by dibutyryl cAMP in these cells. The synthesis of hCG-α and hCG in nontrophoblastic tumor cells was stimulated by dibutyryl cAMP. cAMP and 8-bromo-cAMP, however, did not affect the synthesis of hCG-α, although thesynthesis of hCG was enhanced. 5-Bromo-2′-deoxyuridine induced hCG-α synthesis but did not affect hCG synthesis in nontrophoblastic tumor cells. In HeLa S3 and HeLa 65 cells, the amount of hCG-α and hCG produced in the presence of dibutyryl cAMP and 5- bromo-2′-deoxyuridine together was greater than the total hormone produced by each inducer separately. Our data indicate that the regulation of the production of hCG-α and hCG differs in trophoblastic and nontrophoblastic tumors.

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