Use of Normal Rabbit Serum in Production of Hypercholesteremia in Cholesterol Fed Rats.

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Abstract
Blood was removed (8 cc. per animal) from each of 14 male, 260-g Long-Evans rats. The plasma was discarded and the cells from each separate animal resuspended in 4 cc. of normal rabbit serum. The reconstituted blood was returned to the respective animals. After 3 min. the entire procedure was repeated. Following plasmapheresis 9 were given 100 mg. of cholesterol in olive oil and placed thereafter on a high cholesterol diet. The remaining 5 rats were placed on a cholesterol-free diet. The avg. plasma cholesterol of plasmapheresed rats on a high cholesterol diet rose from 50 mg.% (immediately after plasmapheresis) to 115 mg.% in 48 hrs. The corresponding values for rats on a cholesterol-free diet were 51-68 mg.%, while normal control rats on a high cholesterol diet rose from 59 to 64 mg.%.