Comparison of the Effects of Divalent Ion Chelating Agents on Complement-Mediated Hemolysis in Rabbit and Human Serum
Open Access
- 1 November 1969
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 103 (5) , 1044-1049
- https://doi.org/10.4049/jimmunol.103.5.1044
Abstract
The chelating agent, magnesium ethylene-glycol-diamino-ethyl-tetraacetic acid (MgEGTA), inhibits EAC′ hemolysis when human serum is used as a complement source, but does not inhibit rabbit platelet injury which occurs in the presence of antigen-antibody reactions unrelated to platelet antigens. This difference has been assumed to indicate that the mechanism of complement action in producing this type of immunologically-induced platelet injury differs from that subserving EAC′ hemolysis. The present studies indicate that when rabbit serum rather than human serum is used as a source of complement, EAC′ hemolysis is not inhibited by MgEGTA. It is concluded that rabbit complement and human complement differ with respect to divalent ion requirement. The explanation for this difference may be that the concentration of calcium ion required for maintaining the rabbit C′1 molecule in an assembled state is lower than the concentration required for normal human C′1 activity.Keywords
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