Host Genetic Control of Mouse Hepatitis Virus Type 4 (JHM Strain) Replication. I. Restriction of Virus Amplification and Spread in Macrophages from Resistant Mice

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Abstract
Peritoneal macrophages were used to analyze host genetic control of mouse hepatitis virus type 4 (MHV-4) infection. Infectious center and immunofluorescence assays indicated that only a subset of macrophages from susceptible (BALB, C3H or C57BL/6J) or resistant (SJL/J) mice were initially infected with MHV-4 during the 1st cycle of infection. Compared to macrophages from susceptible mice, 3- to 6-fold fewer SJL/J macrophages were infected, and there was no amplification of virus replication by involvement of adjacent cells during the 2nd cycle of infection. Treatment of macrophages from susceptible mice with interferon-.beta. could not duplicate the aborted 2nd cycle of infection that occurred in macrophages from resistant mice.