Determination of Mugineic Acid, 2′-Deoxymugineic Acid, 3-Hydroxymugineic Acid, and Their Iron Complexes by Ion-Pair HPLC and Colorimetric Procedures

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Abstract
Extract Under iron (Fe)-deficient conditions like in calcareous and/or high pH soils, mugineic acid family phytosiderophores (MAs: mugineic acid (MA), 2′-deoxymugineic acid (DMA), 3-hydroxymugineic acid (HMA) etc.) are secreted from graminaceous plants and solubilize the slightly soluble Fe in soil as MAs-Fe complexes (Takagi 1976, 1993). Due to their high availability to higher plants (Roemheld and Marschner 1986), the behavior of MAs and their Fe complexes in the soil environment is of interest in connection with the iron nutrition of these plants.