Replication of adenovirus DNA in vitro is ATP‐independent
- 3 January 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 337 (1) , 33-38
- https://doi.org/10.1016/0014-5793(94)80624-1
Abstract
Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10‐fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+could efficiently be used for DNA chain elongation on synthetic templates we hypothesize that Mg2+ is essential for the transition of initiation to elongation.Keywords
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