Graded Postischemic Reoxygenation Ameliorates Inhibition of Cerebral Cortical Protein Synthesis in Dogs

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Abstract
The purpose of this study was to determine the effect of normoxic reperfusion and graded postischemic reoxygenation on cerebral protein synthesis in a cell-free system. Ischemia alone produced a relatively small decrease (15–17%) in activity in all the subcellular systems studied. After a 15-min interval of normoxic reperfusion (75–90 mm Hg O2 in arterial blood), a 40% decrease (p < 0.01) in [14C]leucine incorporation was observed. Reoxygenation with hypoxemic blood containing 37.5 mm Hg O2 at 0–5 min and 56 mm Hg O2 at 6–10 min of recirculation followed by 5 min of normoxic reperfusion resulted in a significant increase (p < 0.05) of polypeptide chain synthesis in vitro when compared with normoxic reperfusion. The results obtained by this experimental approach tend to show that graded postischemic reoxygenation could be used as a simple and effective neuroprotective tool that substantially diminishes the secondary postischemic damage in nervous tissue, including the newly synthesized proteins.