HUMAN LEUKOCYTE RESPONSES INVITRO .1. TRANSFORMATION OF PURIFIED T LYMPHOCYTES WITH AND WITHOUT ADDITION OF PARTIALLY PURIFIED MONOCYTES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 29  (2) , 295-303
Abstract
Highly purified Fc receptor-negative T [thymus-derived] lymphocytes were obtained by filtration of human blood mononuclear cells through Ig[immunoglobulin]-anti-Ig columns. Monocyte-enriched cells were isolated by density centrifugation in bovine serum albumin solutions. The proliferative in vitro response of the purified T lymphocytes was investigated with and without the addition of monocyte-enriched cells, after stimulation by allogeneic cells, phytohemagglutinin (PHA), pokeweed mitogen (PWM), purified protein derivative (PPD), Candida albicans, Staphylococcus aureus, Escherichia coli and tetanus toxoid. Fc receptor-negative T lymphocytes responded autonomously and were the main source of proliferative cells found after stimulation by allogeneic cells and optimal doses of PHA, PWM and PPD. Addition of monocyte-enriched cells increased the responses, presumably by a non-specific feeder-effect in the cultures. Stimulation of Fc receptor-negative T lymphocytes by C. albicans, S. aureus, E. coli and tetanus toxoid required the presence of autologous monocytes in the cultures, whereas allogeneic monocytes did not support the responses. Fc receptor-negative T lymphocytes were the main proliferating cells found after stimulation by C. albicans, whereas other cells (B[bone marrow-derived]-lymphocytes and/or Fc receptor-positive T lymphocytes) may have been responsible for a substantial part of the proliferation elicited by S. aureus, E. coli and tetanus toxoid. Fc receptor-negative T lymphocytes can stimulate allogeneic cells in mixed lymphocyte culture, but monocyte-enriched cells and unfractionated mononuclear cells were better in this respect.