False Negative HLA—DR Assignment of Necrokidney Donors. A Glucocorticoid Effect?

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Abstract
Potential necrokidney donors [13] were HLA-DR typed using peripheral blood B [bone marrow-derived] lymphocytes (PBBL). Antigen assignment was impossible in 10 cases because of non-reactivity of the PBBL with the HLA-DR test serum set, in some of the cases in spite of positive reactions with a positive control xenogeneic anti-human B cell antiserum. In 5 of these 10 donors, HLA-DR typing was also performed on B lymphocytes isolated from the spleens, and in all 5 cases strong specific reactions were obtained, allowing reliable antigen assignment. The negative serum reactions with the 5 donors'' PBBL may be falsely negative. Glucocorticoid treatment prior to blood sampling for HLA-DR typing may be responsible for the phenomenon.