Minor components of reduced bovine .KAPPA.-casein.
- 1 January 1979
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 25 (2) , 95-102
- https://doi.org/10.3177/jnsv.25.95
Abstract
Bovine .kappa.-casein reduced with 2-mercaptoethanol was fractionated on a DEAE-cellulose column to 1 nonadsorbed, 5 major adsorbed and 2 minor adsorbed fractions. The properties of the nonadsorbed and 5 major adsorbed fractions were reported in a previous paper. In this paper, the characteristics of 2 minor adsorbed fractions (P-X and P-Y) were reported. Gel electrophoretic patterns of these fractions were similar to .kappa.-casein. These fractions had the same amino acid composition and the same P content as the major components. P-X contained 1 residue each of N-acetylneuraminic acid, galactose and galactosamine, and P-Y 2 residues each. These fractions showed the stabilizing ability for .alpha.sl-casein in the presence of Ca ion. P-X and P-Y are apparently minor components of reduced bovine .kappa.-casein.This publication has 12 references indexed in Scilit:
- Purification and some physicochemical properties of bovine κ-caseinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- Nomenclature of the Proteins of Cow's Milk: Fourth RevisionJournal of Dairy Science, 1976
- Fractionation and Some Properties of κ-Casein VariantsJournal of Dairy Science, 1966
- Chromatographic Isolation and Partial Characterization of Reduced κ-Casein Components*Biochemistry, 1966
- Fractionation of S-Carboxymethyl-κ-casein and characterization of the componentsBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- The heterogeneity of κ-caseinBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- Purification and Some of the Properties of αs-Casein and κ-CaseinJournal of Dairy Science, 1963
- Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoidsBiochemical Journal, 1961
- Phosphorus Assay in Column ChromatographyJournal of Biological Chemistry, 1959