Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (20) , 6025-6029
- https://doi.org/10.1093/nar/18.20.6025
Abstract
A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.This publication has 21 references indexed in Scilit:
- Mixed deoxyribo- and ribo-oligonucleotides with catalytic activityNature, 1990
- Autolytic processing of a phosphorothioate diester bondNucleic Acids Research, 1988
- A small catalytic oligoribonucleotideNature, 1987
- Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sitesCell, 1987
- Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymeraseNucleic Acids Research, 1987
- Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesNucleic Acids Research, 1987
- Structural analysis of spermine and magnesium ion binding to yeast phenylalanine transfer RNA.Proceedings of the National Academy of Sciences, 1978
- A crystallographic study of metal-binding to yeast phenylalanine transfer RNAJournal of Molecular Biology, 1977
- RNA - ligand interactions:(I) magnesium binding sites in yeast tRNAPheNucleic Acids Research, 1977
- Structural Domains of Transfer RNA MoleculesScience, 1976