Turn Geometry for Minimizing Band Broadening in Microfabricated Capillary Electrophoresis Channels
- 1 June 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 72 (14) , 3030-3037
- https://doi.org/10.1021/ac000054r
Abstract
Turns in microfabricated capillary electrophoresis channels generally result in degraded separation quality. To circumvent this limitation, channels were constructed with different types of turns to determine the design that minimizes turn-induced band broadening. In particular, tapered turns were created by narrowing the separation channel width before the start of a turn and widening the channel after the turn is complete. The radius of curvature of the turn, the length over which the channel is tapered, and the degree of tapering were explored. The column efficiencies were determined by examining the resolution of the 271/281 base pair doublet in the separation of a φX174 HaeIII DNA sizing ladder. Tapered turns with the smallest radius of curvature (250 μm), the shortest tapering length between the separation and turn widths (55 μm), and the largest tapering ratio (4:1 separation channel width to turn channel width) produced the highest resolution separations. These results are discussed by comparison to theoretical predictions of the effect of tapers and turns on analyte band dispersion in capillary electrophoresis.Keywords
This publication has 19 references indexed in Scilit:
- Federal research publishing houses?Analytical Chemistry, 1999
- DNA Sequencing on Microfabricated Electrophoretic DevicesAnalytical Chemistry, 1998
- Capillary Electrophoresis Chips with Integrated Electrochemical DetectionAnalytical Chemistry, 1998
- High-Speed DNA Genotyping Using Microfabricated Capillary Array Electrophoresis ChipsAnalytical Chemistry, 1997
- Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis DeviceAnalytical Chemistry, 1996
- Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis ChipsAnalytical Chemistry, 1995
- Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.Proceedings of the National Academy of Sciences, 1994
- High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis DeviceAnalytical Chemistry, 1994
- Capillary electrophoresis and sample injection systems integrated on a planar glass chipAnalytical Chemistry, 1992
- Planar chips technology for miniaturization and integration of separation techniques into monitoring systemsJournal of Chromatography A, 1992