Identification and Characterization of Ribosomal Proteins Phosphorylated in Vaccinia-Virus-Infected HeLa Cells

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Abstract
Two-dimensional analysis of 32P-labeled ribosomal proteins revealed 3 proteins which are phosphorylated in vaccinia-virus-infected HeLa cells. All 3 proteins belong to the 40-S ribosomal subunits and were identified as S2, S6 and S16. The ribosomal protein S6 is phosphorylated also in uninfected HeLa cells. Phosphoserine was detected in all 3 proteins, phosphothreonine only in the protein S2. Phosphorylation of these ribosomal proteins in infected cells is dependent on the multiplicity of the viral infection and increases during the first 6 h of infection. All 3 proteins are also phosphorylated in virus-infected cells treated with cycloheximide and in cells infected with UV-irradiated virus. The phosphorylation reaction may involve a vaccinia virion-associated protein kinase.