Human Antibody Fragments Specific for Human Blood Group Antigens from a Phage Display Library
- 1 October 1993
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 11 (10) , 1145-1149
- https://doi.org/10.1038/nbt1093-1145
Abstract
We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.Keywords
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