Gα11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin‐releasing hormone in Xenopus oocytes
- 28 July 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 307 (2) , 237-240
- https://doi.org/10.1016/0014-5793(92)80775-c
Abstract
Xenopus oocytes that express mouse thyrotropin‐releasing hormone receptors (TRH‐Rs) after injection if RNA transcribed from TRH‐R cDNA respond to THR by a depolarizing current. This response is transducer by activation of phosphoinositide‐specific phospholipase C and utilizes an as yet unidentified endogenous guanine nucleotide‐binding regulatory (G) protein(s). The α subunit of G11 and Gq have recently been shown to couple receptors to activation of phospholipase C. To determine whether there are functional differences between these proteins, we have co‐expressed the TRH‐R with either α11 or αq, α11 potentiated the response to TRH (by 61±16%), while αq inhibited the response (by 37±9%). The changes in amplitudes were accompanied by inverse changes in response latencies. These data show that α11 and αq differentially modulate signal transduction in Xenopus oocytes.Keywords
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