Antiviral Effect of Interferon Covalently Bound to Sepharose
- 1 August 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (8) , 2360-2363
- https://doi.org/10.1073/pnas.70.8.2360
Abstract
Interferon, covalently bound to Sepharose 4B activated by cyanogen bromide, induces the antiviral state in sensitive cells. The antiviral effect is neutralized by antiserum specific to interferon and is recovered thereafter when the antibody is detached from the interferon by treatment at low pH. Binding interferon to Sepharose increases the stability of the molecule. It is likely that the interferon molecule acts on the cell receptor without being detached from the beads. However, the data do not exclude the possibility of a small loss of interferon, or fragments of it, after contact with the cell.Keywords
This publication has 10 references indexed in Scilit:
- [Inhibition of interferon activity by ouabain].1973
- Mechanism of Interferon Uptake in Parental and Somatic Monkey-Mouse Hybrid CellsProceedings of the National Academy of Sciences, 1973
- Regulation of hepatic glycogen synthetase of Rana catesbeiana. Inhibition of the action of insulin by ouabain.1972
- Biologically Active Catecholamines Covalently Bound to Glass BeadsProceedings of the National Academy of Sciences, 1972
- INTERACTION OF INSULIN WITH THE CELL MEMBRANE: THE PRIMARY ACTION OF INSULINProceedings of the National Academy of Sciences, 1969
- [Study of the mechanism of the refractory state of interferon producing cells, after repeated induction].1968
- Interferon Binding: The First Step in Establishment of Antiviral ActivityScience, 1967
- Antigenic Identity of Interferons induced by Different Viruses in the same Cell SystemNature, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951