A Draft Sequence of the Rice Genome ( Oryza sativa L. ssp. indica )
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- 5 April 2002
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5565) , 79-92
- https://doi.org/10.1126/science.1068037
Abstract
We have produced a draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp. indica , by whole-genome shotgun sequencing. The genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. Functional coverage in the assembled sequences was 92.0%. About 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. Although 80.6% of predicted Arabidopsis thaliana genes had a homolog in rice, only 49.4% of predicted rice genes had a homolog in A. thaliana . The large proportion of rice genes with no recognizable homologs is due to a gradient in the GC content of rice coding sequences.Keywords
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