In vitro and in vivo studies of bovine parvovirus proteins

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Abstract
Total cytoplasmic RNA from bovine parvovirus (BPV)-infected bovine fetal lung cells or BPV-specific RNA selected by hybridization to cloned BPV genomic sequences were translated in a message-dependent rabbit reticulocyte lysate. Immunoprecipitation, using IgG from rabbits injected with purified BPV, resulted in the detection of [35S]methionine-labeled polypeptides with MW of 80,000, 72,000, 62,000 and 60,000. These in vitro translation products had the same mobility on sodium dodecyl sulfate-polyacrylamide gels as that of the 4 proteins found in purified virions. The 3 largest polypeptides had amino acid sequence homology, as judged by serological methods and partial proteolysis with Staphylococcus aureus V8 protease. Additional noncapsid proteins with MW of 25,000, 27,000 and 31,000 were also detected as translation products of these RNA. All of the above species were immunoprecipitated by IgG from a calf which was naturally infected with BPV. All 4 capsid proteins but only 1 of the lower MW polypeptides were detected after the immunoprecipitation of BPV-infected cells. Evidently, the BPV genome codes for four capsid proteins and a noncapsid protein which may be structurally related to the capsid proteins.