Differences in induction of sister chromatid exchanges in MNNG-resistant Hela S3 Mer−cell lines
- 1 July 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 11 (7) , 1209-1212
- https://doi.org/10.1093/carcin/11.7.1209
Abstract
All N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-resistant (MR) cell lines of HeLa S3 Mer− cells tested were found to be defective in O6-methylgunine-DNA-methyltransferase and more sensitive than HeLa Mer+ cells to 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-1-nitroso-urea, like the parental HeLa S3 Mer− cells. MR10-1 amd MR1-3 cells were also highly resistant to induction of sister chromatid exchanges (SCEs) by MNNG, while MR6, MR8 and MR11 cells were slightly resistant to SCE induction. MR10-1 and MR1-3 cells showed higher spontaneous mutation rates than the parental cells. These results indicated that there are at least two types of MR cells. All MR cells were more sensitive than HeLa S3 Mer− cells to 6-hydroxy-amino purine.This publication has 26 references indexed in Scilit:
- Efficient correction of mismatched bases in plasmid heteroduplexes injected into cultured mammalian cell nuclei.Molecular and Cellular Biology, 1985
- Correlation between O6-methylguanine-DNA-methyltransferase activity and resistance of human cells to the cytotoxic and mutagenic effect of N-methyl-N′-nitro-N-nitrosoguanidineCarcinogenesis: Integrative Cancer Research, 1984
- Comparison of repair of O6-methylguanine produced by N-methyl-N'-nitro-N-nitrosoguanidine in mouse and human cellsCarcinogenesis: Integrative Cancer Research, 1984
- RELATIONSHIP OF DNA-REPAIR PHENOTYPES OF HUMAN FIBROBLAST AND TUMOR STRAINS TO KILLING BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE1984
- SENSITIVITY OF HUMAN CELL STRAINS HAVING DIFFERENT ABILITIES TO REPAIR O-6-METHYLGUANINE IN DNA TO INACTIVATION BY ALKYLATING-AGENTS INCLUDING CHLOROETHYLNITROSOUREAS1984
- FORMATION OF THE CROSS-LINK 1-[N-3-DEOXYCYTIDYL],2-[N-1-DEOXYGUANOSINYL]-ETHANE IN DNA TREATED WITH N,N'-BIS(2-CHLOROETHYL)-N-NITROSOUREA1982
- Removal of O6-methylguanine from DNA of normal and xeroderma pigmentosum-derived lymphoblastoid linesNature, 1981
- DNA cross-linking and monoadduct repair in nitrosourea-treated human tumour cellsNature, 1980
- Isolation and characterization of dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12Molecular Genetics and Genomics, 1980
- Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.Proceedings of the National Academy of Sciences, 1980