ISOLATION OF A GLYCOPROTEIN ANTIGEN FROM ASCITES-FLUID OF PANCREATIC CARCINOMA

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (5) , 1525-1529
Abstract
A glycoprotein antigen from the ascites fluid of [human] pancreatic carcinoma was isolated with the use of perchloric acid extraction and chromatographies on Sepharose 4B, DEAE-Sephadex, and DE52, followed by Sephadex G-200. The purified glycoprotein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis. MW of 185,000 daltons was determined by gel filtration. The molecule contained 55% protein and 45% carbohydrate. Both the amino acid and carbohydrate compositions were different from those of carcinoembryonic antigen. This glycoprotein antigen of pancreatic cancer cross-reacted with anticarcinoembryonic antigen; the antiserum prepared from this antigen did not react with carcinoembryonic antigen.

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