Abstract
The effect of different amounts of starch and crude fat on humin formation during the hydrolysis of gluten prepared from a commercial flour depended on the acid/protein ratio, high ratios giving less humin nitrogen and less humin weight. The nitrogen content of humin was almost independent of the acid/protein ratio but increased with decreasing humin formation. Filtration time increased markedly with increasing purity of the samples. The color of the hydrolyzates varied slightly with impurities and acid/protein ratios.

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