Purification of Red Cell Enzymes by Treatment with n-Butanol and Chloroform
- 31 December 1975
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 6 (2-3) , 147-152
- https://doi.org/10.1080/00327487608061609
Abstract
Treatment of packed human red cells with 15% n-butanol and 6% CHCl3 at 5°C denatured 99% of the proteins absorbing at 410 nm. Seven of the twelve red cell enzymes studied were recovered in good yield and with high specific activity.This publication has 16 references indexed in Scilit:
- ERYTHROCYTE METABOLISM. VI. SEPARATION OF ERYTHROCYTE ENZYMES FROM HEMOGLOBIN*Journal of Clinical Investigation, 1962
- The Purification and Mechanism of Action of Yeast PhosphoglucomutaseJournal of Biological Chemistry, 1959
- TRANSAMINASE ACTIVITY IN HUMAN BLOODJournal of Clinical Investigation, 1955
- Acid phosphatase. II. Purification of human red cell phosphomonoesteraseArchives of Biochemistry and Biophysics, 1954