The effect of aqueous surfactant solutions on alcohol dehydrogenase (LADH)
Open Access
- 5 January 1993
- journal article
- review article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 41 (1) , 156-161
- https://doi.org/10.1002/bit.260410120
Abstract
Alcohol dehydrogenase (LADH) was studied in aqueous solutions of surfactants to determine its structural and catalytic characteristics. Fluorescence, circular dichroism (CD), and electron paramagnetic resonance (ERP) techniques were used to study structural changes to the enzyme. The activity of LADH in catalyzing the oxidation of ethanol was investigated. Short‐chain alkyl sulfonates and sulfates did not deactivate LADH or alter its structure. Longer and branched alkyl sulfates and sulfonates, as well as a cationic surfactant (CTAB), affected both LADH activity and conformation. © 1993 John Wiley & Sons, Inc.Keywords
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