Seven linkage groups were identified in A. flavus by means of the parasexual cycle. Twenty genetic markers were assigned to these linkage groups which contained 6, 4, 4, 2, 2, 1 and 1 markers, respectively. Parasexual crosses between markers in each of the 7 linkage groups, 21 possible combinations in all, showed independent assortment. Crosses between markers assigned to the same linkage group indicated a low frequency of mitotic crossing-over.