Foam separation of DNA and proteins from solutions
- 1 October 1982
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 24 (10) , 2253-2262
- https://doi.org/10.1002/bit.260241011
Abstract
Foam separation of bovine serum albumin-DNA and lysozyme-DNA systems is performed. The separation of the total protein from DNA is evaluated for dissociated [calf thymus] chromatin solution. Foam separation for the same systems is done also by a new method of creating a pressure gradient in the Plateau-Gibbs borders in the foam and obtaining a dry foam. The effectiveness of the foam separation can be improved significantly by the application of the latter method. Some factors (pH, initial concentration of the solution, expansion factor of the foam) influencing the separation of proteins from DNA in the foam and in the residual solution are studied as well.This publication has 13 references indexed in Scilit:
- Proteins at liquid interfaces: III. Molecular structures of adsorbed filmsPublished by Elsevier ,2004
- Proteins at liquid interfaces: I. Kinetics of adsorption and surface denaturationPublished by Elsevier ,2004
- Concentration of proteins by foamingBiotechnology & Bioengineering, 1981
- Drainage and stability of foams from aqueous protein solutions when a pressure is applied in their Plateau-Gibbs bordersColloid and Polymer Science, 1979
- Investigation of protein foams obtained by bubblingColloid and Polymer Science, 1979
- Proteins at liquid interfacesJournal of Colloid and Interface Science, 1979
- FoamsPublished by Springer Nature ,1973
- The separation and purification of enzymes through foamingAnalytical Biochemistry, 1966
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956