AN ANALYSIS OF THE EFFECT OF CARBON MONOXIDE ON THE RESPIRATION OF FROG SKELETAL MUSCLE

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Abstract
The fact that CO stimulates rather than inhibits the respiration of frog muscle and that this effect is due to oxidation of the CO to CO2 has been confirmed, and data bearing on the possible mechanism obtained. The oxidation is not confined to resting muscle and proceeds in the presence of lactate, pyo-cyanin, KCl, o-chlorophenol indophenol, p-phenylene di-amine, Nadi reagent, or caffeine, and probably with dextrose and methylene blue. Apparently the CO inhibits the cyto-chrome-cytochrome oxidase system in many of these cases but the superimposed burning of CO often masked the inhibition. In the case of caffeinized muscle this was demonstrated by analysis of the gases used. The oxidation of CO is accomplished by an enzyme system separable from both the cytochrome system and that responsible for the resting respiration. Cyanide, azide, and hydroxylamine prevent CO burning; the latter 2 completely inhibit in concs. that exert no effect on the respiration of resting muscle and but a small effect on the extra respiration of caffeinized muscle.

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