Elevated plasma levels of F2α isoprostane in cystic fibrosis

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Abstract
Cystic fibrosis (CT) is associated with chronic lung infection’ inflammation’ and elevated indices of oxidative stress. Recently’ isoprostanes were shown to be a reliable in vivo marker of oxidant injury with 8‐iso‐PGF2α’ shown to cause airflow obstruction and plasma exudation in guinea pig lung. The present study was designed to examine the relationship between 8‐iso‐PGF2α levels’ plasma antioxidants’ and clinical status in CF. We hypothesized that plasma 8‐iso‐PGF2α levels would be higher in subjects with CT compared to healthy controls. Plasma 8‐iso‐PGF2α levels were prospectively measured in 22 subjects with CF and nine healthy controls using an 8‐isoprostane enzyme immunoassay kit along with plasma vitamins A’ E’ and β‐carotene. Plasma 8‐iso‐PGF2α levels were shown to be significantly elevated in the CE subjects compared to controls (319.6±52.6 vs. 145.0±21.0 pg/ml’ P=0.005). Plasma levels of antioxidants were significantly lower for the CF subjects compared to the controls (vitamin A’PPPiso‐PGF2α levels.