Identification of the Trimannosyl-Chitobiose Structure in Sugar Moieties of Japanese Quail Ovomucoid1

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Abstract
Ovomucoid isolated from egg white of japanese quail contained mannose, galactose, glucosamine, and sialic acid (8.6: 1.3: 6.6:0.5 mol/mol) as sugar components. Sugar chains were liberated from the polypeptide portion by hydrazinolysis, N -acetylated and fractionated on Bio-Gel P-2. The reducing ends of the thus obtained sugar moieties were pyridylaminated. Chemical and exoglycosidase digestion studies of the fluorescent pyridylamino derivative of a major sugar fraction indicated the following structure for one of the major sugar chains of japanese quail ovomucoid; Manal →6(Manal →3)Manβl → 4GlcNAcβl → 4GlcNAc.

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