Kinetics of Transcription in a Minute Column
Open Access
- 1 April 1996
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (7) , 1380-1381
- https://doi.org/10.1093/nar/24.7.1380
Abstract
Immobilized enzymes or nucleic acids are widely used to analyze association and dissociation reactions. A pseudo-rapid kinetic method for studying transcription was developed by using an immobilized template DNA packed in a minute column, in a micro-scale liquid chromatography system. This method, which has a dead-time of only several seconds, is quick enough to allow analysis of the release of product RNA in transcription by Escherichia coli RNA polymerase. The method could be applied to most enzymes that interact with DNA.Keywords
This publication has 7 references indexed in Scilit:
- A Branched Pathway in the Early Stage of Transcription byEscherichia coliRNA PolymeraseJournal of Molecular Biology, 1996
- Recycling of the general transcription factors during RNA polymerase II transcription.Genes & Development, 1995
- Requirement for the .beta.,.gamma.-pyrophosphate bond of ATP in a stage between transcription initiation and elongation by Escherichia coli RNA polymeraseBiochemistry, 1991
- Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template baseJournal of Molecular Biology, 1987
- Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.Proceedings of the National Academy of Sciences, 1981
- Abortive initiation and long RNA synthesisBiochemistry, 1981
- Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoterBiochemistry, 1980