Estimating Immature Populations of Rice Weevils in Wheat by Using Subsamples1

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Abstract
Wheat was infested at three levels with rice weevil (Sitophilus oryza (L.)), differing in age. Each level of infestation was replicated three times. Each replicate was subdivided Six times and one division sampled four times when the organism was in a particular immature form. Eventually all six divisions were sampled. Immature infestation was determined by counting adult emergence from samples. Various factors affecting emergence were studied by a mixed classification (factorial, nested) analysis of variance. It showed: (1) age of cultures used had no effect Oil total emergence; (2) level of infestation accounted for more than 63% of total variation in the experiment; (3) that greater efficiency could be obtained by using a single source of infested wheat; and (4) that consistency can be improved by taking samples for control and treatment from a population that contains a relatively high percentage of one stage. A table useful in estimating the number of samples necessary to obtain reliable estimates of immature infestation is given.

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