Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations
- 4 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 292 (1-2) , 159-161
- https://doi.org/10.1016/0014-5793(91)80857-y
Abstract
A typical hydrophilic enzyme, CT, can be dissolved in nonpolar organic solvents (n‐octane, cyclohexane and toluene) up to μM concentrations. In the homogeneous solution obtained, the enzyme possesses catalytic activity and enormously high thermostability. It does not lose this activity even after several hours refluxing in octane (126°C) or cyclohexane (81°C).Keywords
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