EFFECT OF CRYOPROTECTORS ON STABILITY OF LYSOSOMES UNDER COOLING

  • 1 January 1980
    • journal article
    • research article
    • Vol. 52  (5) , 573-576
Abstract
The efficiency of the cryoprotectors polyethylene oxide 400, glycerol, oxyethylated glycerol and dimethyl sulfoxide was studied relative to stabilization of isolated lysosomes under different regimes of freezing-thawing. A decrease in the amount of lysosomal hydrolases (RNase, DNase, cathepsin D, phosphatase) released to the environment is the most significant in the presence of these cryoprotectors under slow freezing-thawing.