Chromatography of oxidized and reduced cytochrome c on carboxymethylcellulose

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Abstract
Cytochrome c was isolated from horse heart by a chromatographic method. Oxidized and reduced cytochrome c were chromatographed on CM-cellulose that was in equilibrium with several buffer systems of constant composition at pH values of 8.4, 6.75 and 4.9. Separation was better at the higher pH values; the oxidized form was retarded more than twice as much as the reduced form, though they differed by only a single charge. Self-competition between cytochrome molecules is suggested to account for the peak distortion observed at high loads (above 20 [mu]M protein concentration).

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