Pattern of segregation of chicken HPRT phenotype in Chinese hamster-chick red blood cell hybrids
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 24 (3) , 129-137
- https://doi.org/10.1159/000131368
Abstract
The pattern of segregation of hypoxanthine phosphoribosyltransferase (HPRT, E.C. 2.4.2.8) was determined in synchronized Chinese hamster-chick red blood cell hybrids. Three hybrid lines were synchronized at the Gl-S boundary. Bromodeoxyuridine pulses were subsequently applied throughout the S phase, and the frequency of the segregant clones was determined. It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block.Keywords
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