Purification of Rickettsiales-like organisms associated with Pecten maximus (Mollusca Bivalvia): serological and biochemical characterization

Publisher Website
Google Scholar
Abstract
Slgnificant mortalities have been observed in populations of Sainl-Jacques scallops PActen maximus, in association with numerous intrace1lular colonies of Rickettsiales-like organisms (RLO) in the gi1ls. Isolation of the procaryotes from infecled tissues and their subsequent purification wprp achieved by a specifie protocol based on differenlial and isopycnic centrifugations on sucrose and renografin density gradients. The purity and integrity of purified procaryotes were verified by electron microscopy. Specifie polyclonal antibodies were prepared for serological characterization. Fifteen major constituent proteins were eiectrophoretically identified, their molecular weights ranging between 148 and 16 kD. Ten enzyme activities were biochemica1ly identified, including acid phosphatase and catalase, which could be related to procaryote pathogemcity. Slgnificant mortalities have been observed in populations of Saint-Jacques scallops PActen maximus, in association with numerous intrace1lular colonies of Rickettsiales-like organisms (RLO) in the gi1ls. Isolation of the procaryotes from infecled tissues and their subsequent purification wprp achieved by a specifie protocol based on differential and isopycnic centrifugations on sucrose and renografin density gradients" The purity and integrity of purified procaryotes were verified by electron microscopy. Specifie polyclonal antibodies were prepared for serological characterization" Fifteen major constituent proteins were eiectrophoretically identified, their molecular weights ranging between 148 and 16 kD. Ten enzyme activities were biochemica1ly identified, including acid phosphatase and catalase, which could be related to procaryote pathogemcity.

This publication has 0 references indexed in Scilit: