Oligonucleotide probes detect splicing variants insituinDrosophilaembryos

1 January 1992

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 20 (21) , 5687-5690
https://doi.org/10.1093/nar/20.21.5687

Abstract

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.

Keywords

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