Evidence for the Presence of Lectins with Mannose Specificity in the Rat Cerebellum

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Abstract
Two different methods were used to detect the possible presence of lectin-like molecules with a specificity for mannose-rich glycans in the rat cerebellum. The 1st, affinity histochemistry, involved the isolation of a particular class of glycoproteins form the cerebella of 11-day-old rats followed by the formation of covalent complexes with horseradish peroxidase and then incubation with cerebellar slices. The 2nd used in vitro interactions between [3H]leucine-labeled proteins, kept in solution, with insolubilized [14C]glucosamine-labeled glycoproteins. The results of both methods are compatible with the presence of lectin-like activities inhibited by high mannose concentrations, but not other sugars. The binding sites preferred by these molecules seem to be more than a single mannose residue.

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