Practical Nuclear Staining Procedures for Rhizoctonialike Fungi

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Abstract
An HCl-Giemsa staining procedure and 2 rapid direct staining methods were used to stain nuclei of multinucleate and binucleate Rhizoctonia-like fungal isolates. Isolates were cultured on thin layers of Difco potato dextrose agar (DPDA) in plastic petri plates. Disks cut from the cultures were transported through the HCl-Giemsa staining process in tissue carrier mounts. Stained disks were mounted in dark corn syrup. The DPDA cultures also were stained directly in the plates with either 0.5% aniline blue or 0.05% trypan blue in lactophenol. In either case, the mycelium to be stained first was wet with an acidified wetting agent to facilitate staining. Cover slips were added and the stained hyphae were microscopically examined in the plates with a dry .times. 40 objective. Of the staining methods tried HCl-Giemsa consistently gave better results.