Impact of Competitive Inhibition and Sequence Variation upon the Sensitivity of Malaria PCR
- 1 May 2007
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (5) , 1621-1623
- https://doi.org/10.1128/jcm.02145-06
Abstract
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.Keywords
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