An Oxygen-Dependent Postirradiation Restoration of Rhizopus Stolonifer Sporangiospores

Abstract
Inhibition of germination after normally lethal doses of gamma irradiation resulted in a striking recovery of the colony-forming potential of R. stolonifer sporangiospores. Germination was prevented by concentrating the spores into a dense suspension (5.6 X 107/ml) in water. During the postirradiation period, low concentrations sufficed, but no recovery occurred without oxygen in the absence of an exogenous substrate. Recovery in spore suspensions in equilibrium with atmospheres of 1 or 2% approached recovery in 100% oxygen atmospheres. When germination was effectively inhibited, a temperature near optimum for growth favored recovery.

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