Alkali treatment for rapid preparation of plant material for reliable PCR analysis
Open Access
- 16 March 1993
- journal article
- Published by Wiley in The Plant Journal
- Vol. 3 (3) , 493-494
- https://doi.org/10.1111/j.1365-313x.1993.tb00169.x
Abstract
For plant genetics, it would be useful to monitor easily the segregation of different alleles using the polymerase chain reaction (PCR). Preparation of DNA templates from individual plants needs to be rapid and reliable. A one tube protocol is described that involves subjecting plant tissue pieces to alkali, neutralization and heat denaturation prior to PCR analysis, and that proved to be much faster and more reliable than published protocols.Keywords
This publication has 2 references indexed in Scilit:
- Direct amplification of plant genomic DNA from leaf and root pieces using PCRPlant Molecular Biology, 1991
- A simple and rapid method for the preparation of plant genomic DNA for PCR analysisNucleic Acids Research, 1991