Sympathetic nervous system and renin release from submaxillary glands in vitro

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Abstract
[It was previously reported that .alpha.- but not .beta.-adrenergic agonists stimulate renin release from mouse submaxillary glands in vivo.] This study was undertaken to determine if these in vivo effects were due to a direct action on the submaxillary glands and to find out if cyclic AMP (cAMP) might be involved in submaxillary renin release. Pooled mouse submaxillary gland slices were incubated in Krebs-Ringer bicarbonate medium following a preincubation period, and renin release was measured by a radioimmunoassay for the direct measurement of submaxillary gland renin. Tissue cAMP levels were measured. Addition of the .alpha.-adrenergic agonists, phenylephrine or norepinephrine, significantly increased renin release (P < 0.01 vs. control) while decreasing tissue cAMP levels (P < 0.01 vs. control). Addition of the .beta.-adrenergic agonist isoproterenol markedly increased cAMP levels (P < 0.01 vs. control) and decreased renin release (P < 0.05 vs. control). Pretreatment of the slices with the .alpha. blocker phenoxybenzamine inhibited the effect of phenylephrine. .alpha.-Adrenergic agonists may cause renin release from submaxillary glands which is accompanied by a fall in tissue cAMP levels. This is in contrast to renin release from the kidney which is stimulated by .beta.-adrenergic agonists.

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