Further experiments on biological differences between natural sea waters

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Abstract
In one experiment sea water from the Firth of Clyde yielded better cultures of Echinus esculentus larvae than did sea waters from two positions in the English Channel.From the Clyde water materials of unknown composition were extracted, using active carbon and acetone. The addition of these materials to both Clyde and Channel waters resulted in the death of the Echinus eggs during early cleavage. A similar extraction from Channel water had the same effect. The Clyde and Channel waters from which these materials had been extracted gave poor abnormal cultures, especially the Channel water in which most larvae died early. A few, however, survived, and later on some of these grew into almost normal plutei.
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