Simple Butyrate Esterase Stain for Monocytes

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Abstract
The esterases used to identify [human] monocytes are best demonstrated using .alpha.-naphthyl butyrate as substrate. The reagents commonly used for this stain are time-consuming to prepare and are unstable. A quick, easy and reproducible staining method was described, using stable reagents which are readily available commercially but which may also be prepared in the laboratory. [The use of this method for the differential diagnosis of monocytic leukemia, easily confused with acute myeloid leukemia, was discussed].

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