THE BACTERIOPHAGY OF A SPORE-FORMING ORGANISM

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Abstract
The isolation of 2 bacteriophages specific for Bacillus megatherium is described. 7 bacterial strains, obtained from different sources, were susceptible to the lytic action. Each bacteriophage contained 2 principles, 1 of which was characterized by ability to diffuse from the initial plaque and cause changes in the surrounding culture. Spores seem to be resistant to the action of bacteriophage, but are inhibited from developing by its presence. The lytic principles were destroyed by exposure to 75[degree] C. for 10 min., and could in this way be eliminated from cultures containing organisms in the spore stage. The lytic process as seen under the micro-scope is described. Bacillus megatherium does not, apparently, swell or burst in lysis, but rather fades from view.

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