Intensification of antitumor-immunity by protein-bound polysaccharide, EA6, derived from Flammulina velutipes (CURT. ex FR.) SING. combined with murine leukemia L1210 vaccine in animal experments.

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Abstract
The antitumor effect of protein-bound polysaccharide, EA6, derived for fruit bodies of F. velutipes when combined with a vaccine treatment was studied by the challenge test in BDF1 mice and L1210 murine leukemia system. Intensification of the antitumor effect of EA6 was dependent on doses, timing and frequency of i.p. administration of EA6 to the immunization by concanavalin A- and/or glutaraldehyde-treated L1210 vaccine. Administration of EA6 prior to the injection of the vaccine or repeated injection > of 4 times did not increase the life-span of the animals. When EA6 was given (40 mg/kg) after the injection of the vaccine, marked prolongation of the life-span (ILS [increase in life-span over control] of 223%) was observed against challeging of 1 .times. 102 cells of L1210. Combined treatment of EA6 with vaccine exhibited prolonged ILS in the mice challenged with 1 .times. 103 cells of L1210. The specific immunity for L1210 induced by the vaccine was not affected by EA6.