Kinetics of bilirubin oxidation with peroxidase, as applied to studies of bilirubin - albumin binding

Abstract
In the determination of unbound bilirubin by rate of oxidation with peroxidase, errors may be caused by (1) phenol, propylparaben, and phenothiazines (free radical acceleration), (2) haemoglobin (peroxidase effect), and (3) ascorbate (inhibition). Such errors may be diminished by dilution 1:40, or with an antioxidant, tert-butyl-p-hydroxyanisole, and ascorbate oxidase.