STIMULATION BY METHOTREXATE OF HUMAN CHORIONIC-GONADOTROPIN AND PLACENTAL ALKALINE-PHOSPHATASE IN CULTURED CHORIOCARCINOMA CELLS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (12) , 4570-4576
Abstract
Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a 10-fold increase in synthesis of human chorionic gonadotropin and a 2-fold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous addition of thymidine or folinic acid, neither of which alone increases human chorionic gonadotropin synthesis or placental alkaline phosphatase activity in BeWo cells.